Jurkat cells were left unstimulated (left) or stimulated for 20 hours with Anti-Human CD95 (APO-1/Fas) Functional Grade Purified coated at 5 µg/mL in a 24-well culture plate (right).The stimulated cells were then harvested and stained sequentially with Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) and the Annexin V Apoptosis Detection Kit eFluor® 450 (Product # 88-8006-72), then fixed and permeabilized with the Foxp3 Staining Buffer Set (Product # 00-5523-00) and stained with Anti-Human PARP1 (Cleaved) PE (Product # 12-6668-42).Total viable cells (Fixable Viability Dye eFluor® 780 negative) were used for analysis.